熊本大学 生命資源研究・支援センター 動物資源開発研究部門
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動物資源開発研究施設(CARD) > 研究室 > 資源開発分野 > 実験動物関係教職員高度技術研修 >CARD - KRIBB TRAINING COURCE FOR REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE

CARD - KRIBB TRAINING COURCE FOR REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE


CARD - KRIBB TRAINING COURCE FOR REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE was held in Daejeon 28th-30th October 2015.
Group Photo1
Group Photo2

28 October, 2015

9:00 Arrival and Registration of participants
Welcome Address
9:30 Introductory lecture
10:00 1. IVF between fresh sperm and fresh or cryopreserved oocytes
10:30 Sperm harvesting
11:00 Oocyte harvesting and preparation
11:30 Insemination
12:00 Demonstration of removal of cumulus cells from oocytes
12:30 Lunch
13:30 Making pipette
14:00 Embryo handling
14:30 Oocyte washing
15:00 Coffee break
15:30 Sperm cryopreservation
17:00 Oocyte observation
18:00 Welcome dinner (including participants)

29 October, 2015

9:30 2. IVF between frozen-thawed sperm and fresh oocytes
10:00 Oocyte harvesting
10:30 Sperm thawing
Insemination
11:00 3. IVF using sperm cryopreserved by Jackson method
11:30 Oocyte harvesting
Sperm thawing
12:00 Insemination
12:30 Lunch
13:30 Embryo handling and observation from Day 1
14:00 Lecture: The CARD by Prof. Nakagata
14:30 Oocyte washing
15:00 Coffee break
15:30 Demonstration of cold storage of cauda epididymides
16:00 Demonstration of cold storage of embryos
16:30 Lecture: The theory of CARD protocol in mouse reproductive technology by Dr. Toru Takeo
17:00 Lecture: Current status and activity of LACR at KRIBB by Dr. Ki-Hoan Nam
17:30 Oocyte observation

30 October, 2015

9:30 Demonstration of superovulation using IAS
10:30 Embryo handling and observation from Day 2
11:00 Embryo vitrification
11:30 Embryo warming
12:30 Lunch
13:30 Embryo transfer
15:00 Demonstration of collection of the cold-stored epididymides
15:30 Demonstration of collection of the cold-stored embryos
16:00 Demonstration of surgical operation for preparing vasectomized mice
16:30 Review course, Results, Concluding remarks, Diplomas
18:00 Farewell party