熊本大学 生命資源研究・支援センター 動物資源開発研究部門
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動物資源開発研究部門(CARD) > 研究室 > 資源開発分野 > 実験動物関係教職員高度技術研修 > CARD-CNB Mouse Sperm and Embryo Cryopreservation Course in Spain

CARD-CNB Mouse Sperm and Embryo Cryopreservation Course in Spain


CARD-CNB Mouse Sperm and Embryo Cryopreservation Course was held in Spain on 7th-11th October 2013.

24 participants from 16 countries (Australia, Brazil, Canada, Czech
Republic, Denmark, Finland, France, Israel, Italy, the Netherlands, Portugal, Spain, Sweden, Taiwan, the UK, and the USA) took part in the course.
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There, various lectures were given and many techniques were explained by doctors. These techniques were then demonstrated by CARD-CNB staff and practiced by participants. Practical training and exciting discussions were carried out throughout all three steps.

While the 24 participants were practicing, 8 technical instructors were standing by them to help and reply to any questions or requests.

All problems were resolved quickly and the course was completed on schedule. Thanks to the organization and cooperation of staff from CSIC-CNB in Spain, the course successfully came to a close.

We are confident that many professionals from all over the world acquired valuable experience on the course. Their results can be found here.

7 October, 2013

9:00 Registration of Participants
9:30 Welcome address
Carmen Castresana and Lluis Montoliu
10:00 Introduction of the course
Naomi Nakagata
11:00 Coffee break
11:30 New EU Directive on animal experimentation
Belen Pintado
12:15 The Guide for the Care and Use of Laboratory Animals and the AAALAC Accreditation
Javier Guillen
13:00 Lunch at CNB Cafeteria
14:00 Epigenetic effects of in vitro culture of embryos
Alfonso Gutierrez-Adan
15:00 History and Fundaments of Cryobiology
Jorge Sztein
16:00 Comparing current embryo and sperm cryopreservation methods
Jorge Sztein
17:00 Visiting the CNB and the lab room
Julia Fernandez and Lluis Montoliu

8 October, 2013

8:30 Training: Preparing drops of FERTIUP PM for fresh sperm and CARD medium for IVF (Demonstration only)
9:00 Training: Obtaining sperm from males
10:00 Training: Collecting oocytes from females and insemination (IVF)
11:00 Coffee break
11:30 Training: Preparing embryo handling device and pipettes
Training: Practice for embryo handling
12:30 Questions and Answers
13:00 Lunch at CNB Cafeteria
14:00 Training: Washing embryos and incubating in HTF O/N
14:30 Training: Cold-storage of epididymides
15:30 Safety and hazards handling liquid nitrogen
Jesus Martinez
16:00 Training: Preparing drops for sperm cryopreservation and sperm cryopreservation
17:00 Training: Observing pronucleus formation in fertilized oocytes

9 October, 2013

8:30 Thawing sperm and collecting sperm from cold-stored epididymes
9:00 Training: Collecting oocytes from females and insemination (IVF)
10:30 Training: Observing embryos from day 2
11:00 Coffee break
11:30 Training: Cold storage of embryos
12:30 Questions and Answers
13:00 Lunch at CNB Cafeteria
14:00 Training: Washing embryos and incubating in HTF O/N
14:30 Training: Freezing 2-cell embryos using BIOCOOL (controlled rate freezing)
16:00 Questions and Answers
16:30 Training: Abdominal vasectomy and escrotal vasectomy
17:00 Training: Observing pronucleus formation in fertilized oocytes

10 October, 2013

8:30 Observing embryos from day 3
9:00 Class on vitrification.
Naomi Nakagata
9:30 Training: Vitrification of 2-cell embryos and oocytes
11:00 Coffee break
11:30 Training: Thawing vitrified oocytes and 2-cell embryos
12:30 Training: Thawing 2-cell embryos frozen with BIOCOOL
13:00 Lunch at CNB Cafeteria
14:00 Training: Collection of cold-stored 2-cell embryos
14:30 Questions and Answers
15:30 Training: Embryo transfer into oviducts and demonstration of ICSI
20:00 Course Official Dinner down town in Madrid

11 October, 2013

8:30 Observing embryos from day 2 and day 3
Selecting late morulas and blastocysts
9:30 NSET videos / talk
Barbara Stone
10:00 Learning and practising embryo transfer into uterus and NSET
11:00 Coffee break
11:30 Questions and Answers
12:00 EMMA and INFRAFRONTIER
Lluis Montoliu
12:30 Overview of ZFN, TALEN and CRISPR in transgenic animals
Kay Schonig
13:00 Lunch at CNB Cafeteria
14:00 Shipping mice, frozen or refrigerated embryos/sperm across the world
Toru Takeo
14:30 The CARD
Naomi Nakagata
15:00 Databases in archiving centres
Shuji Tsuchiyama
15:30 Questions and Answers
General Discussion on any topic of the course
16:30 Concluding remarks
Diplomas
Lluis Montoliu and Naomi Nakagata
.