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動物資源開発研究施設(CARD) > 研究室 > 資源開発分野 > 実験動物関係教職員高度技術研修 > CARD - RPCI Cryopreservation Workshop

CARD - RPCI Cryopreservation Workshop


CARD - RPCI Cryopreservation Workshop was held in Buffalo on 15th-19th September 2014.
・Pre - course information
・The course report

18 participants from 8 countries (Australia, Canada, France, Germany, New Zealand, Saudi Arabia, The UK, and The USA) took part in the course (Picture).

15 September, 2014

8:00~8:45 Registration of participants
8:45~9:00 Welcome Address: Aimee Stablewski and Jan Parker-Thornburg
9:00~9:45 Introduction to the workshop: Naomi Nakagata
9:45~10:30 History and fundamentals of cryopreservation: Jorge Sztein
10:30~11:00 Coffee Break
11:00~11:45 Comparing current embryo and sperm cryopreservation methods: Lluis Montoliu
11:45~12:15 Vitrification of oocytes and their use for IVF: Naomi Nakagata
12:15~13:15 Lunch
13:15~13:45 IACUC and training issues: Sandra Sexton
14:00~16:00 Cold storage of cauda epididymis and sperm freezing
16:00~17:00 CRISPR/Cas9 and Gene Editing Endonucleases: Lluis Montoliu

16 September, 2014

8:30~9:00 Preparing dishes
9:00~12:00 Collection of fresh oocytes
Obtaining of fresh sperm, frozen sperm and cold-stored sperm
IVF using fresh oocytes and fresh sperm
IVF using fresh oocytes and frozen sperm
IVF using fresh oocytes and cold-stored sperm
12:30~13:45 Lunch
14:00~15:00 Washing
15:30~16:30 Taconic Lecture
17:00~17:30 Observation of pronuclear development and scoring

17 September, 2014

8:30~10:30 Oocyte vitrification
10:30~11:00 Observation and collection of 2-cell embryos from fresh, frozen and cold-stored sperm IVF
11:00~12:00 Vitrification of the 2-cell embryos
12:00~13:00 Lunch
13:00~14:30 Thawing of the 2-cell embryos
Cold storage of the vitrified-warmed 2-cell embryos
14:40~15:00 Development of database for managing mouse banking system: Shuuji Tsuchiyama
15:30 Bus to Niagara Falls

18 September, 2014

8:30~9:00 Collection of fresh oocytes
Thawing of vitrified oocytes
9:00~10:00 Thawing of JAX/CARD sperm
Collection of fresh sperm
10:00~11:00 IVF using fresh oocytes and JAX/CARD sperm
IVF using vitrified oocytes and fresh sperm
11:00~12:00 Collection of cold-stored embryos
12:00~13:00 Lunch
13:00~13:30 Washing
13:30~14:00 Lecture on ovary freezing: Jorge Sztein
14:00~16:00 Ovary freezing
Thawing of ovaries and implantation
16:00~16:30 Observation of pronuclear development and scoring
16:30~17:00 Review of sperm analyzed data: Nancy Mutch

19 September, 2014

8:30~9:00 Observation and collection of 2-cell embryos from JAX/CARD IVF and vitrified oocytes IVF
9:00~10:30 Embryo transfer through the wall of oviduct
10:30~12:00 Embryo transfer via NSET
12:00~13:00 Lunch
13:00~13:30 Shipping mice, frozen or refrigerated embryos/sperm across the world: Toru Takeo
13:30~14:30 General discussion of the course
14:30~15:00 Results, Concluding remarks, diplomas