Reproductive Engineering Techniques in Mice, Second Edition

New contents

• Removing one Cauda Epidydimis from an Anesthetized Male
• Preparation of laser-microdissected Oocytes
• Transport of 2-Cell Embryos at Cold Temperature
　

Revised contents

• Preparing and Assembling Pipettes for Embryo Handling
• In Vitro Fertilization
• Cryopreservation of Mouse Spermatozoa
• In Vitro Fertilization using Cryopreserved Spermatozoa
• Collecting Two-Cell Stage Embryos
• Vasectomy for the Creation of Sterile Males
• Embryo Transfer into Oviduct
• EmbryoTransfer into the Uterus
　

Center for Animal Resources & Development,
Kumamoto University,
card3gpo.kumamoto-u.ac.jp

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