Division of Reproductive Engineering

Introduction of Mouse Bank Service in CARD, Kumamoto University

Databases of mice strains in CARD (R-base)

• The informations of our databases are available at the International Mouse Strain Resource (IMSR) databases also.
• CARD has currently collected following.(15 October 2015)

Order live population or frozen embryos

• Application procedure
• Poster.pdf

Online manuals

• Collection and Transport at Cold Temperature of Cauda Epididymis
• In Vitro Fertilization using Epidydimal Sperm Transported at Cold Temperature
• In Vitro Fertilization (IVF)
• In Vitro Fertilization (IVF) using Superovulation Reagent
• Embryo Transfer into the Oviduct
• Cryopreservation of Mouse Spermatozoa
• Cryopreservation of Mouse Spermatozoa using a Cryotube
• In Vitro Fertilization using Cryopreserved Spermatozoa
• Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa
• Simple Vitrification of Mouse Embryos
• Procedure for Warming of Vitrified Embryos
• Simple Vitrification of Mouse Oocytes
• Transport of 2-Cell Embryos at Cold Temperature
• Storage of Media and Solutions in Ampoules Under Nitrogen Gas
• Tables of Media Composition
  • 1M DMSO - DAP213 - 0.25M Sucrose - HTF - KSOM/AA - CARD-IASe

Training courses

• 2015 October CARD - KRIBB TRAINING COURCE FOR REPRODUCTIVE ENGINEERING TECHNIQUES IN MICE
• 2014 September CARD - RPCI Cryopreservation Workshop was held in Buffalo
• 2013 October CARD-CNB Mouse Sperm and Embryo Cryopreservation Course in Spain
• 2013 May Cryopreservation and Reproduction Techniques for Laboratory Mouse in Beijing
• 2012 November Cryopreservation and Reproduction Techniques for Laboratory Mouse in Taiwan
• 2011　December Workshop on Mouse Embryo Freezing The 7th SALAS Regional Conference in conjunction with the 6th AMMRA Council Meeting in Singapore
• 2008 April Training course in Korea
• 2005 June Training course in Pekin
• 2002 June Training course in Shanghai

Publications

• Publications

Center for Animal Resources & Development,
Kumamoto University,
cardkumamoto-u.ac.jp

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