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Mice health management


At the Animal Facility, animal care managed monitor mice are used to monitor the health of all mice at mice rooms.


Care and examination of monitor mice

By inspecting and caring for the monitor mice using the method below, the health of all mice in the animal care rooms can be monitored.
  1. The air flow of animal care rooms is controlled and designed so that air flows into monitor cages after passing through other cages.
  2. When cages are changes during animal care management, a small amount of feces from animal care cages in the mice rooms is added to monitor mice cages.
  3. Monitor mice raised for more than 10 weeks are then examined.














Mice examination items


　Pathogenic microorganisms that have been identified with respect to monitor mice are shown below. (2015/10/02)
Examination items examination method
Mice
Rats
Ectromelia virus
 ELISA/IFA
○
−
Lymphocytic Choriomeningitis Virus
 ELISA/IFA
○
−
Mouse adenovirus
 ELISA/IFA
○
○
Mouse hepatitis virus
 ELISA/IFA
○
−
Pneumonia virus of mice
 ELISA/IFA
○
○
Sendai virus
 ELISA/IFA
○
○
Sialodacryoadenitis virus
 ELISA/IFA
−
○
CAR  bacillus
ELISA/IFA
○
○
Citrobacter rodentium
culture; DHL agar culture medium
(caecal content)
○
−
Clostridium piliforme
 ELISA/IFA
○
○
Corynebacterium kutscheri
culture; FNC culture medium
(trachea, laryngopharynx, caecal content)
○
○
Helicobacter hepaticus
CR (feces)
○
−
Mycoplasma pulmonis
 ELISA/IFA/
culture; PPLO broth broth
(trachea, laryngopharynx)
○
○
Salmonella typhimurium
 culture; DHL agar medium
(caecal content)
○
○
★Pseudomonas aeruginosa
 culture; NAC agar medium
(caecal content)
○
○
★Pasteurella pneumotropica
 culture; blood sample agar medium
(trachea/laryngopharynx)
○
○
★Staphylococcus aureus
 culture; mannitol with vitellus,
Salt culture medium(caecal content)
○
○
Aspiculuris tetraptera
naked-eye observation of imago
(proximal portion of colon)
○
−
Syphacia obvelata
 microscopic examination; observation of eggs using cellophane tape method (perianal region)
○
−
Syphacia muris
 microscopic examination; observation of eggs using cellophane tape method (perianal region)
−
○
Giardia muris
microscopic examination; direct smear sample (duodenal content)
○
○
Spironucleus muris
 microscopic examination; direct smear sample (duodenal content)
○
○
Trichomonas spp.
microscopic examination; direct smear sample (caecal content)
○
○
Ectoparasite
microscopic examination; observation of imago using cellophane tape method, observation of eggs (fur)
○
○
ELISA：enzyme-linked immunosorbent assay、IFA：Indirect fluorescent antibody method、
★: immunodeficient animals only



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