Animal management

 Regarding animal management methods, please refer to the following.

 Staff manage experimental animals to prevent infection with disease so that correct experimental data can be obtained quickly.

Animal care area divisions

 The Animal Facility is divided as shown below. The isolation level for pathogenic microorganisms is different for each area.
 The area on the right has a high isolation level and strict care is required in regards to use.
Conventional area < SPF area < Infection experiments area < Isolator chamber

 For details, please refer here.

Animal care management

 Each area in which animals are cared for is kept clean by Animal Facility staff who give food and water, change cages, and apply disinfectant.
 The management policies common to each area are listed below.

  1. Feed
    The feed used is prepared at the Animal Facility. It is replenished weekly by Animal Facility staff when cages are changed.
  2. Water
    UV-treated water is used (for automatic water supply).
  3. Changing cages
    Sterile bedding and cages are used and cages are changed every week (for mice and rats).
    At the same time, animal health is observed and animals are checked for signs of any suffering due to serious infections.
  4. Cleaning
    The animal care room is wiped down with disinfectant weekly at the same time that cages are changed.
    The halls of the Animal Facility are also regularly wiped down.
  5. Environment
    In regards to the environment (such as temperature and humidity) provided by facility equipment, see here for details.
  6. Estimation of animal care expenses
    In order to calculate costs for animal care support by facility staff such as changing cages, etc. (animal care management duties), the number of cages used by researchers is checked daily.

 Consultations regarding changes to animal care style or special feeding methods should be addressed to facility staff or to the following email address.
 ->Contact List (Internal University use only)

Regulation of pathogenic microorganisms


 Infection of facility animals is monitored according to the following steps.
  1. Inspection of monitored animals (for mice)
  2. Initial inspection and quarantine of received and unloaded animals.
  3. Observation of clinical symptoms during care management of large animals
  4. Observation by facility staff during animal care management
  5. Quarantine of cells and biological formulations brought to the Animal Facility

Main inspected items

  Mice Rats Dogs Monkeys Cells Inspection methods
(Inspected area)
Inspection frequency
Mycoplasma pulmonis
Yes Yes ELISAa/IFAb (blood samples), cultures (trachea, laryngopharynx) 12d
Bordetella bronchiseptica
Yes cultures (trachea, laryngopharynx) 4
Brucella canis
Yes cultures (blood) upon arrival only
CAR  bacillus
Yes Yes ELISA/IFA (blood sample) 1
Citrobacter rodentium
Yes cultures (Caecal content) 4
Clostridium piliforme
Yes Yes ELISA/IFA (blood sample) 12
Corynebacterium kutscheri
Yes Yes cultures (trachea, laryngopharynx, Caecal content) 4
Helicobacter hepaticus
Yes PCRc (feces) 4
Pasteurella pneumotropica
Yes cultures (trachea/laryngopharynx, vaginal swab) 4e
Pseudomonas aeruginosa
Yes Yes cultures (Caecal content) 4e
Salmonella spp.
Yes Yes Yes cultures (Caecal content, feces (monkeys)) 4(monkeys: when needed)
Staphylococcus aureus
Yes cultures (Caecal content) 4e
Streptococcus pneumoniae
Yes cultures (trachea/laryngopharynx) 4
B virus
Yes ELISA (blood sample) outsourced upon arrival only
Ectromelia virus
Yes ELISA/IFA (blood sample) 4
Lymphocytic chorimeningitis virus
Yes Yes IFA (blood sample) 1
Mouse adenovirus
Yes Yes ELISA/IFA (blood sample) 4
Mouse hepatitis virus・SDAV
Yes Yes Yes ELISA/IFA (blood sample), PCR (feces, cells) 12
Pneumonia virus of mice
Yes Yes ELISA/IFA (blood sample) 1
Sendai virus
Yes Yes ELISA/IFA (blood sample) 12
Aspiculuris tetraptera
Yes Microscopic examination (colon contents) 12
Syphacia spp.
Yes Yes Microscopic examination (Perianal region) 12
Giardia muris
Yes Yes Microscopic examination
(Duodenal content)
Spironucleus muris
Yes Yes Microscopic examination
(Duodenal content)
Trichomonas  spp.
Yes Yes Microscopic examination (Caecal content) 12
Yes Yes Microscopic examination (fur) 12
Parasite eggs Yes Yes Flotation method, Microscopic examination (feces) when needed
Heartworm microfilaria Yes Microscopic examination (blood) upon arrival only
Dermatophytic fungi Yes Yes cultures (fur) when needed
a: enzyme antibody method, b: indirect fluorescent antibody method, c: polymerase chain reaction, d: times/year, e: immunodeficient mice only

Division in charge

 The Division of Microbiology and Genetics is responsible for inspection of pathogenic microorganisms.
 Please contact the supervisor below with questions regarding health inspection of animals during experiments, health inspection of animals other than mice, disclosure of period inspection results for mice, etc.

 Supervisor: Nakamura
 ->Contact List (Internal University use only)

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Animal experimentation at Kumamoto University
How to use
Regarding experiments with genetically modified organisms
Mice breeding method
Animal management
Regulation of pathogenic microorganisms

Creation of genetically modified mice
Microbial Quality Control
Mouse Bank

Gene Technology Center
Kumamoto University

Kumamoto University
Institute of Resource Development and Analysis
Center for Animal Resources and Development

2-2-1 Honjo, Chuo-ku, Kumamoto-shi
TEL  :096-373-6550
FAX  :096-373-6552
Email :mimura(a)
Contact List (Internal University use only)

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