0.25M sucrose

組成および作成方法

PB1medium

Reagent name	mg/100mL
NaCl	800.0
KCl	20.0
CaCl2	12.0
KH2PO4	20.0
MgCl2・6H2O	10.0
Na2HPO4	115.0
Na-Pyruvate	3.6
Glucose	100.0
Penicillin	7.5
Streptomycin	5.0
BSA	300.0

0.25Msucrose in PB1

Reagent name	mg/20mL in PB1
Sucrose	1711.5

参考文献

Nakao K., Nakagata N., and Katsuki M. 1997. Simple and effcient procedure for cryopreservation of mouse embryos by simple vitrification. Exp. Anim. 46: 231-234.
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