KSOM/AA
組成および作成方法
| Reagent name | mg/100mL |
| NaCl | 555 |
| KCl | 18.5 |
| KH2PO4 | 4.75 |
| MgSO4・7H2O | 4.95 |
| CaCl2・2H2O | 25 |
| NaHCO3 | 210 |
| Glucose | 3.6 |
| Na-Pyruvate | 2.2 |
| Na-lactate(mL) | 0.174 |
| 10mM EDTA(µL) | 100 |
| Penicillin | 6.3 |
| Streptomycin | 5 |
| 0.5％phenol red(mL) | 0.1 |
| L-Glutamine | 14.6 |
| アミノ酸溶液(mL) | 1 |
| 非必須アミノ酸溶液(mL) | 0.5 |
| BSA | 100 |
参考文献
Lawitts J.A., and Biggers J.D. 1993. Culture of preimplantation embryos. Methods Enzymol. 225:153-164.