mHTF
組成および作成方法
| Reagent name | mg/100mL |
| NaCl | 593.8 |
| KCl | 35.0 |
| MgSO4・7H2O | 4.9 |
| KH2PO4 | 5.4 |
| CaCl2 | 57.0 |
| NaHCO3 | 210.0 |
| Glucose | 50.0 |
| Na-lactate (mL) | 0.34 |
| Na-Pyruvate | 3.7 |
| Penicillin G | 7.5 |
| Streptomycin | 5.0 |
| BSA (Albumin,Bovine serum,Fraction V, Fatty Acid-Free) |
400 |
| 0.5％phenol red(mL) | 0.04 |
参考文献
Kito S., Hayao T., Noguchi-Kawasaki Y., Ohta Y., Hideki U., and Tateno S. 2004. Improved in vitro fertilization and development by use of modified human tubal fluid and applicability of pronucleate embryos for cryopreservation by rapid freezing in inbred mice. Comp. Med. 54(5): 564-570.更新履歴
- 新規 : 2012.01.23
- 更新 : 2014.04.22 The reference was changed.