資源開発分野

マウスバンク

生殖工学技術研修会

IRDA
熊本大学

mHTF



組成および作成方法


Reagent name mg/100mL
NaCl 593.8
KCl 35.0
MgSO4・7H2O 4.9
KH2PO4 5.4
CaCl2 57.0
NaHCO3 210.0
Glucose 50.0
Na-lactate (mL) 0.34
Na-Pyruvate 3.7
Penicillin G 7.5
Streptomycin 5.0
BSA
(Albumin,Bovine serum,Fraction V, Fatty Acid-Free)
400
0.5%phenol red(mL) 0.04


参考文献

Kito S., Hayao T., Noguchi-Kawasaki Y., Ohta Y., Hideki U., and Tateno S. 2004. Improved in vitro fertilization and development by use of modified human tubal fluid and applicability of pronucleate embryos for cryopreservation by rapid freezing in inbred mice. Comp. Med. 54(5): 564-570.


更新履歴