mHTF
| 试剂名 | mg/100mL | 供应商 | 目录号 |
| NaCl | 593.8 | Sigma | S5886 |
| KCl | 35.0 | Sigma | P5405 |
| MgSO4 7H2O | 4.9 | Sigma | M7774 |
| KH2PO4 | 5.4 | Sigma | P5655 |
| CaCl2 | 57.0 | Sigma | C5670 |
| NaHCO3 | 210.0 | Sigma | S5761 |
| Glucose | 50.0 | Sigma | G6152 |
| Na-lactate (mL)* | 0.34 | Sigma | L7900 |
| Na-Pyruvate | 3.7 | Sigma | P4562 |
| Penicillin G | 7.5 | Sigma | P4687 |
| Streptomycin | 5.0 | Sigma | S1277 |
| BSA (Albumin, Bovine serum, Fraction V, Fatty Acid-Free) |
400 | MERCK/CALBIOCHEM | 126575 |
| 0.5% phenol red (mL) | 0.04 | Sigma | P0290 |
参考文献
- Kito S., Hayao T., Noguchi-Kawasaki Y., Ohta Y., Hideki U., and Tateno S. 2004. Improved in vitro fertilization and development by use of modified human tubal fluid and applicability of pronucleate embryos for cryopreservation by rapid freezing in inbred mice. Comp. Med. 54(5): 564-570.
更新记录
- 更新 : 1 July, 2018