mHTF
| Reagent Name | mg/100mL | Vendor | Catalog Number |
| NaCl | 593.8 | Sigma | S5886 |
| KCl | 35.0 | Sigma | P5405 |
| MgSO4 7H2O | 4.9 | Sigma | M7774 |
| KH2PO4 | 5.4 | Sigma | P5655 |
| CaCl2 | 57.0 | Sigma | C5670 |
| NaHCO3 | 210.0 | Sigma | S5761 |
| Glucose | 50.0 | Sigma | G6152 |
| Na-lactate (mL)* | 0.34 | Sigma | L7900 |
| Na-Pyruvate | 3.7 | Sigma | P4562 |
| Penicillin G | 7.5 | Sigma | P4687 |
| Streptomycin | 5.0 | Sigma | S1277 |
| BSA (Albumin, Bovine serum, Fraction V, Fatty Acid-Free) |
400 | MERCK/CALBIOCHEM | 126575 |
| 0.5% phenol red (mL) | 0.04 | Sigma | P0290 |
References
- Kito S., Hayao T., Noguchi-Kawasaki Y., Ohta Y., Hideki U., and Tateno S. 2004. Improved in vitro fertilization and development by use of modified human tubal fluid and applicability of pronucleate embryos for cryopreservation by rapid freezing in inbred mice. Comp. Med. 54(5): 564-570.
Update history
- Modified : 22 April, 2014
The reference was changed.