Introduction

Nakagata laboratory searches mouse reproduction.
This web site has the practical procedures of them.

These techniques can contribute 3Rs and accelerate biomedical research.

Contents

• Collection and Transport at Cold Temperature of Cauda Epididymis
• In Vitro Fertilization using Epidydimal Sperm Transported at Cold Temperature
• In Vitro Fertilization (IVF)
• In Vitro Fertilization (IVF) using Ultra-Superovulation Reagent ( Fertilized oocyte )
• In Vitro Fertilization (IVF) using Ultra-Superovulation Reagent ( From 2-cell to Blastcyst stage embryo )

• Embryo Transfer into the Oviduct

• Cryopreservation of Mouse Spermatozoa
• Cryopreservation of Mouse Spermatozoa using a Cryotube
• In Vitro Fertilization using Cryopreserved Spermatozoa
• Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa

• Simple Vitrification of Mouse Embryos
• Procedure for Warming of Vitrified Embryos
• Simple Vitrification of Fertilized Oocytes
• Simple Vitrification of Mouse Oocytes

• Transport of 2-Cell Embryos at Cold Temperature

• Storage of Media and Solutions in Ampoules Under Nitrogen Gas
• Tables of Media Composition
  • 1M DMSO - DAP213 - 0.25M Sucrose - mHTF - KSOM/AA - CARD HyperOva

How to master this?

Nakagata laboratory holds the training course of the techniques. Please refer to the web site below.