KSOM



Reagent Name mg/100ml Vendor Catalog Number
NaCl 555 Sigma S 5886
KCl 18.5 Sigma P 5405
KH2PO4 4.75 Sigma P 5655
MgSO4 7H2O 4.95 Sigma M 7774
CaCl2 2H2O 25 Sigma C 7902
NaHCO3 210 Sigma S 5761
Glucose 3.6 Sigma G 6152
Na-Pyruvate 2.2 Sigma P 4562
DL-Lactic Acid, sodium salt 0.174ml Sigma L 1375
10mM EDTA 100μl Sigma E-6635
Streptomycin 5 Sigma S 9137
Penicillin 6.3 Sigma P 7794
0.5% phenol red 0.1ml Sigma P 0290
L-Glutamine 14.6 Sigma G 8540
MEM Essential Amino Acids 1ml Gibco 11130-051
MEM Non-essential AA 0.5ml Sigma M 7145
BSA 100 Sigma A-4378



References

  1. Lawitts J.A., and Biggers J.D. 1993. Culture of preimplantation embryos. Methods Enzymol. 225:153-164.